To be honest, FlowJo has always had a bit of trouble Piece explicitly specified), and Gating-ML 2.0 (which is mainly based on an Gating-ML 1.5 (which transforms data using compound transformation with Transformation workflow is implemented is completely different between > spectrumMatrix (manually or by altering the source code), but the result Gating-ML 2.0 (as summarized in pages 91-93 ofįlowcyt.sf.net/gating/latest.pdf). There are several other important changes between Gating-ML 1.5 and On Tue, at 4:15 PM, Mike Jiang here is the answer from I think it still applies, but let me know if you need any more details. You can mention that Gating-ML 2.0 is now an official ISAC standard (i.e., approved by ISAC council on January 13, 2015). Maybe try to ping them to check what their estimated timeline may be. I believe FlowJo's committed to improve their Gating-ML support, including proper support for Gating-ML 2.0 with the spectrumMatrix element (eventually). ![]() To be honest, FlowJo has always had a bit of trouble working with Gating-ML transforms, so even if you got a proper Gating-ML 1.5 output, FlowJo wouldn't likely reproduce the gate memberships properly. Most importantly, the way how the transformation workflow is implemented is completely different between Gating-ML 1.5 (which transforms data using compound transformation with piece explicitly specified), and Gating-ML 2.0 (which is mainly based on an implicit workflow). You could change the spilloverMatrix -> spectrumMatrix (manually or by altering the source code), but the result would be far from Gating-ML 1.5. There are several other important changes between Gating-ML 1.5 and Gating-ML 2.0 (as summarized in pages 91-93 of flowcyt.sf.net/gating/latest.pdf). Sorry, flowUtils cannot write Gating-ML 1.5 output
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Precious pear tart Almond frangipane & ginger nut crustĢ40g soft unsalted butter, plus extra for greasing These concerns get hype especially when there is an app. There has already been a lot of suspicions on the data privacy initiatives of Google backup. Google Backup and Sync Encryption of User Data. For business level usage, Google has introduced Drive File Stream. Tip: After you install ‘Google Drive’, you can right-click on any local folder you want to sync and select ‘Sync or Backup this Folder’. It must be noted that Google Backup and Sync are exclusively for personal usage. 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Brontë’s protagonist is an outcast with questionable bloodlines and questionable social origins. Ruthven was intelligent, sophisticated, and of course, deadly.Ĥ) Emily Brontë: Wuthering Heights (1847) By the mid-1800s, England was deep in the Victorian Era and Gothic literature was the rage. His vampire was not the shuffling revenant of Eastern European legends. Born during the famous “weekend” at Lord Byron’s, Polidori created the aristocratic, sinister, brooding shadow of evil that we know so well today. It’s a fascinating examination of dark desires, sin, evil, and corruption of the soul.ģ) John Polidori: The Vampyre, A Tale (1819) Although technically a short story, Polidori’s story has become the foundation for the modern vampire legend. He meets a mad prince who serves as his double throughout the story. A monk is seduced into ingesting a powerful elixir and he begins experiencing intense sensual desires. It explores the concept of the doppelgänger, a German term for double. Hoffmann: The Devil’s Elixirs (1815) Originally published in Germany, this novel was inspired by an earlier work, The Monk. She challenges the reader to abandon all notions of morality, social convention, and structure throughout this frightening look into power, control, and a god-like existence run amok.Ģ) E.T.A. Man created fire and now Man has created life. He seizes the very core power reserved for the deities alone. Her protagonist becomes God when he creates life from inanimate flesh. This was a time of scientific exploration swathed in the fear and awe of religion. All are equally worthy.ġ) Mary Shelley: Frankenstein (1818) Shelley’s novel is still one of the most powerful novels of the Gothic period. There is no particular order to the list other than sheer numerical for organization. The best Gothic fiction is an arguable list indeed. They exposed those elements of ourselves we begged to remain hidden. They explored the sinister, hidden regions of humanity. They delved into the darkness of the human soul. I make jest of course, as these authors and many others forged a bold new form of literature. People like Ann Radcliffe, Clara Reeve, Friedrich Schiller, and Mathew Gregory Lewis wrote a few novels. In the 1800s, this guy named Lord Byron wrote a few poems. Towering spires that climb to the heavens. It was a building style popular in the high medieval period. Gothic began as a term to describe architecture. Goths dress in black, wear a lot of makeup, and listen to Bauhaus. Gothic is a term that has been usurped by our modern world. Hill Jr.īook Review for The Demon of Decay by Alex C. Gates Richard Farren Barber, The Living and The Lost reviewīook Review for iHunt: Killing Monsters in the Gig Economy by David A. 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Third poem: Roll The Dice ( Text version) This one shows how we should go all the way when we try something. She had on a see-through blouse with a very brief brassiere. She was standing in front of the Bran Flakes and the Wheaties, skirt about 3 inches above the knee and tight-fitting. They can heal with their touch, they can kill what they touch and I am dying but not dead yet. At the age of three, he came with his family to the United States and grew up in Los Angeles. Upstairs a man pukes his entire stomach into a wastebasket. Bukowski wrote thousands of poems, hundreds of short stories and six novels, eventually publishing over sixty books Charles Bukowski was the only child of an American soldier and a German mother. Browse all poems and texts published on Charles BukowskiHis poetry was influenced by the social, cultural, and economic ambience of his home city of Los. In this list, you will find a list of his most beautiful and famous poems. Wive's heads are battered against kitchen walls by unemployed butchers. Bukowski wrote thousands of poems, hundreds of short stories and six novels, eventually publishing over sixty books. It’s moving the sun as you put your shoes on for the last time without knowing it.Īnd I hear the sound of high heels biting into the pavement outside.īorn to sever fingers on the edge of tuna cans. The red moon whirls, a mouse runs along the windowsill changing colors, I am alone in torn levis and a white sweatshirt –– she’s with her man now in the shadow of that wall and as he enters her I draw upon my cigarette. Hell crawls through the window without a sound, enters my room, takes off his hat and sits down on the couch across from me. There is loneliness in this world so great that you can see it in the slow movement of a clock’s hand. She’s more dangerous than all the armies of all time. It is hard to find a man whose poems don’t finally disappoint you. ![]() My typewriter at this hour would scream like a raped bear. When things get bad enough, the kitten will kill the lion.ĭeath comes slowly like ants to a fallen fig. I held my head in all its tender precious agony and we drank together. In the sun and in the rain, in the day and in the night, pain is a flower, pain is flowers, blooming all the time.Īll people start to come apart finally and there it is: just empty ashtrays in a room or wisps of hair on a comb in the dissolving moonlight. He could write a fiery sentence, and with using words that a man of little education could recognize –– this, for me, will forever be a sign of literary genius. begins and ends with the single sentence. doesn’t have to be complicated and that it 2). 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